Evaluation of the accuracy of various phenotypic tests to detect oxacillin resistance in coagulase-negative staphylococci

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Evaluation of the accuracy of various phenotypic tests to detect oxacillin resistance in coagulase-negative staphylococci.

In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30 microg-cefoxitin and 1 microg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. ...

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Is the cefoxitin disk test reliable enough to detect oxacillin resistance in coagulase-negative staphylococci?

Coagulase-negative staphylococci (CoNS) represent an important etiology of nosocomial bloodstream infections (2, 4, 6). The introduction of empirical treatment has been a crucial step in reducing morbidity and mortality caused by such infections and in controlling the spread of resistant strains (1, 6). It has been advocated that the cefoxitin disk would be more sensitive than the 1g oxacillin ...

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Evaluation of oxacillin and cefoxitin disks for detection of resistance in coagulase negative staphylococci.

Coagulase-negative Staphylococcus spp. was considered nonpathogenic until the emergence of multiresistance and the demonstration of their participation as infectious agents. In Brazil, oxacillin resistance may be present in over 80% of isolates, and the Clinical and Laboratory Standards Institute standardized a disk-diffusion method to predict this resistance in Staphylococcus. The aim of this ...

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Performance of different phenotypic tests in detecting mecA mediated methicillin resistance in Coagulase negative staphylococci (CoNS)

Accurate detection of methicillin resistance (MR) in coagulase negative staphylococci (CoNS) is of utmost importance as infections due to CoNS are on the rise. Various phenotypic methods have been described for detecting mecA mediated MR with varying performance characteristics. The present study was designed to evaluate the performance of oxacillin and cefoxitin disc diffusion / MIC test and o...

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Resistance of Coagulase-positive Staphylococci to Methicillin and Oxacillin.

Gravenkemper, Charles F. (University of Washington School of Medicine, Seattle), Jean L. Brodie, and William M. M. Kirby. Resistance of coagulase-positive staphylococci to methicillin and oxacillin. J. Bacteriol. 89:1005-1010. 1965.-Two strains resistant to methicillin were discovered among 541 strains of Staphylococcus aureus isolated in a clinical laboratory during a 1-yr period, and their pr...

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ژورنال

عنوان ژورنال: Brazilian Journal of Infectious Diseases

سال: 2008

ISSN: 1413-8670

DOI: 10.1590/s1413-86702008000300009